ANTICOAGULANT POTENTIAL OF POLYSACCHARIDE ISOLATED FROM THE INDIAN RED ALGA, Asparagopsis taxiformis (Delile) Trevisan

作者：Manilal Aseer^*; Sujith Sugathan; Selvin Joseph; Panikkar Mamkoottahil Velayudhan Nataraja; George Shiney

来源：Thalassas: An International Journal of Marine Sciences , 2012, 28(2): 9-15.

• Anticoagulant
• Asparagopsis taxiformis
• red algae
• seaweeds
• sulfated polysaccharide

A sulfated polysaccharide with anticoagulant activity was purified from the red alga Asparagopsis taxiformis (Delile) Trevisan in Bonnemaisoniaceae family. The molecular mass of the purified polysaccharide ranged between 60 and 500 KD. The anticoagulant activity of the purified compound was assayed using APTT (Activated partial thromboplatin time) and PT (Prothrombin time) assays. Commercial heparin was used as standard to determine the relative potency of the sulfated polysaccharide isolated from A. taxiformis. The purified polysaccharide had a relative clotting factor of 28.57 (>1000s +/- 3.6) at 48 mu g/mL, the value was comparable with heparin at 60 mu g/mL. Statistical analysis revealed that there was no significant difference (p>0.05) between the anticoagulant activity of the purified polysaccharide and commercial heparin. Based on the present findings, it was envisaged that the polysaccharide from A. taxiformis might be capable of inhibiting both intrinsic and extrinsic pathways of blood coagulation. The relative clotting factor assayed by PT was within the range of the oral anticoagulant agent. Therefore, the sulfated polysaccharide of A. taxiformis could be developed as a potential oral anticoagulant agent.

• 出版日期2012-7