Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein

作者：Richter Dennerlein Ricarda; Oeljeklaus Silke; Lorenzi Isotta; Ronsoer Christin; Bareth Bettina; Schendzielorz Alexander Benjamin; Wang Cong; Warscheid Bettina; Rehling Peter^*; Dennerlein Sven

来源：Cell, 2016, 167(2): 471-+.

DOI：10.1016/j.cell.2016.09.003

Mitochondrial ribosomes translate membrane integral core subunits of the oxidative phosphorylation system encoded by mtDNA. These translation products associate with nuclear-encoded, imported proteins to form enzyme complexes that produce ATP. Here, we show that human mitochondrial ribosomes display translational plasticity to cope with the supply of imported nuclear-encoded subunits. Ribosomes expressing mitochondrial-encoded COX1 mRNA selectively engage with cytochrome c oxidase assembly factors in the inner membrane. Assembly defects of the cytochrome c oxidase arrest mitochondrial translation in a ribosome nascent chain complex with a partially membrane-inserted COX1 translation product. This complex represents a primed state of the translation product that can be retrieved for assembly. These findings establish a mammalian translational plasticity pathway in mitochondria that enables adaptation of mitochondrial protein synthesis to the influx of nuclear-encoded subunits.

• 出版日期2016-10-6