3,5-Diiodo-L-Thyronine Affects Structural and Metabolic Features of Skeletal Muscle Mitochondria in High-Fat-Diet Fed Rats Producing a Co-adaptation to the Glycolytic Fiber Phenotype

作者：Silvestri Elena; Cioffi Federica; De Matteis Rita; Senese Rosalba; de lange Pieter; Coppola Maria; Salzano Anna M; Scaloni Andrea; Ceccarelli Michele; Goglia Fernando; Lanni Antonia; Moreno Maria^*; Lombardi Assunta^*

来源：Frontiers in Physiology, 2018, 9: 194.

DOI：10.3389/fphys.2018.00194

• skeletal muscle
• proteomics
• mitochondrion
• diiodothyronine
• reactive oxygen species
• inflammation
• mitochondrial dynamics
• adipose differentiation-related protein

Hyperlipidemic state-associated perturbations in the network of factors controlling mitochondrial functions, i.e., morphogenesis machinery and metabolic sensor proteins, produce metabolic inflexibility, insulin resistance and reduced oxidative capacity in skeletal muscle. Moreover, intramyocellular lipid (IMCL) accumulation leads to tissue damage and inflammation. The administration of the naturally occurring metabolite 3,5-diiodo-L-thyronine (T2) with thyromimetic actions to high fat diet (HFD)-fed rats exerts a systemic hypolipidemic effect, which produces a lack of IMCL accumulation, a shift toward glycolytic fibers and amelioration of insulin sensitivity in gastrocnemius muscle. In this study, an integrated approach combining large-scale expression profile and functional analyses was used to characterize the response of skeletal muscle mitochondria to 12 during a HFD regimen. Long-term 12 administration to HDF rats induced a glycolytic phenotype of gastrocnemius muscle as well as an adaptation of mitochondria to the fiber type, with a decreased representation of enzymes involved in mitochondrial oxidative metabolism. At the same time, T2 stimulated the activity of individual respiratory complex I, IV and V. Moreover, 12 prevented the HFD-associated increase in the expression of peroxisome proliferative activated receptor gamma coactivator-1 alpha and dynamin-1-like protein as well as mitochondrial morphological aberrations, favoring the appearance of tubular and tethered organelles in the intermyofibrillar regions. Remarkably, 12 reverted the HDF-associated expression pattern of proinflammatory factors, such as p65 subunit of NF-kB, and increased the fiber-specific immunoreactivity of adipose differentiation related protein in lipid droplets. All together, these results further support a role of T2 in counteracting in vivo some of the HFD-induced impairment in structural/metabolic features of skeletal muscle by impacting the mitochondrial phenotype.

• 出版日期2018-3-9