Apoptosis and autophagy control cell proliferation in the dentate gyrus following hippocampal lesion

作者：Zhou, Ju; Peng, Wei; Zhu, Qi; Gong, Shan; Shan, Lidong; Hisamitsu, Tadashi; Guo, Shiyu; Jiang, Xinghong^*

来源：Neural Regeneration Research, 2010, 5(20): 1541-1547.

DOI：10.3969/j.issn.1673-5374.2010.20.003

• cell proliferation
• neurogenesis
• dentate gyrus
• apoptosis
• autophagy
• programmed cell death
• neural regeneration

Brain injuries often result in the promotion of cell proliferation in the hippocampal dentate gyrus (DG), but the number of newborn cells declines with time. However, the cause of this decline remains poorly understood. Elucidation of the fate of these newborn cells will further the understanding of the pathological process and treatment of brain injury. In the present study, the number of newborn cells was quantitatively analyzed using an unbiased stereological method following hippocampal lesion by kainic acid, in combination with detection of apoptosis and autophagy. Results revealed that hippocampal lesion resulted in a significantly increased number of 5-bromo-2-deoxyuridine (BrdU)-positive cells in the DG, which subsequently decreased with time. BrdU/cleaved caspase-3 double-labeled cells were detected in the granular cell layer and hilus of DG. However, expressions of LC3-II, Beclin 1, and p53 were upregulated, and pro-caspase-3 and Bcl-2 were downregulated. Results indicated that hippocampal lesion in adult rats resulted in significant cell proliferation in the DG, which subsequently reduced with time. In addition, results suggested that apoptosis and autophagic processes could regulate cell proliferation in the DG following hippocampal lesion.

• 出版日期2010-10-30
• 单位苏州大学