科研之友
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摘要
Highly dispersed gold nanoparticles (AuNPs) were introduced into a hierarchically porous zeolite of the MFI type that contains mesopores and an inherently microporous structure. These represent a novel matrix for the immobilization of biomolecules. The composites were characterized by FTIR, X-ray diffraction, UV-vis spectroscopy, transmission electron microscopy, nitrogen sorption measurements, and electrochemical impedance spectroscopy. The crystallinity and morphology of the zeolite is not compromised by incorporating the AuNPs with their size of 3-20 nm. A sensor for hydrogen peroxide (H2O2) was fabricated by incorporating hemoglobin into the matrix and placing it on the surface of a glassy carbon electrode. The resulting biosensor exhibits excellent bioelectrocatalytic capability for the reduction of H2O2. The amperometric response at -0.4 V linearly depends on H2O2 in the 1.0 mu M to 18 mM concentration range. The detection limit is 0.8 mu M (at an S/N of 3). Its good sensitivity, stability and reproducibility make the modified hierarchically porous zeolite a promising new matrix material for protein immobilization and the construction of biosensors.
