Actinoplanes utahensis ZJB-08196 fed-batch fermentation at elevated osmolality for enhancing acarbose production

作者：Wang, Ya-Jun; Liu, Li-Ling; Wang, Yuan-Shan; Xue, Ya-Ping; Zheng, Yu-Guo^*; Shen, Yin-Chu

来源：Bioresource Technology, 2012, 103(1): 337-342.

DOI：10.1016/j.biortech.2011.09.121

• Acarbose
• Fed-batch
• Actinoplanes utahensis
• Maltose
• Osmolarity

Acarbose, a potent alpha-glucosidase inhibitor, is as an oral anti-diabetic drug for treatment of the type two, noninsulin-dependent diabetes. Actinoplanes utahensis ZJB-08196, an osmosis-resistant actinomycete, had a broad osmolality optimum between 309 mOsm kg(-1) and 719 mOsm kg(-1). Utilizing this unique feature, an fed-batch culture process under preferential osmolality was constructed through intermittently feeding broths with feed medium consisting of 14.0 g l(-1) maltose, 6.0 g l(-1) glucose and 9.0 g l(-1) soybean meal, at 48 h, 72 h, 96 h and 120 h. This intermittent fed-batch culture produced a peak acarbose titer of 4878 mg l(-1), increased by 15.9% over the batch culture.

• 出版日期2012-1
• 单位浙江工业大学