Allosteric Regulation in Gating the Central Channel of the Nuclear Pore Complex

作者：Koh Junseock^*; Blobel Guenter

来源：Cell, 2015, 161(6): 1361-1373.

DOI：10.1016/j.cell.2015.05.013

The nuclear pore complex (NPC) arose in evolution as the cell's largest and most versatile transport channel. Current models for selective transport mediated by NPCs are focused on properties of intrinsically disordered regions of nucleoporins that bind transport factors. In contrast, structured regions are considered to provide static anchoring sites for the disordered regions without affecting transport factor binding. Here, we demonstrate allosteric coupling between a structured domain of a channel nucleoporin (Nup58) and its neighboring disordered domain in interaction with another channel nucleoporin (Nup54) and a transport factor (Kap beta 1). Analysis of multiple equilibria showed that multivalent interactions of Kapb1 with the disordered domains of Nup58 stabilize the neighboring structured domain associated with Nup54, shifting conformational equilibria from homo-oligomers to hetero-oligomers. Based on these and previous crystallographic results, a quantitative framework was established to describe constriction and dilation of the central channel as a function of transport factor occupancy.

• 出版日期2015-6-4