Neuronal expression of inducible nitric oxide synthase in hypothyroid rat

作者：Xu, Jialu; Xu, Qin; Chen, Xian; Zou, Chaochun; Zhao, Zhengyan^*

来源：Neuroendocrinology Letters, 2010, 31(6): 848-851.

• inducible nitric oxide synthase
• hypothyroidism
• neuronal development
• real-time polymerase chain reaction
• Western blot

OBJECTIVES: The expression of iNOS mRNA and protein levels was studied in hypothyroid rat cerebral cortex during early days after postnatal life.
METHOD: The homogenate of the cerebral cortex was examined using Real-time reverse transcription polymerase chain reaction (real-time RT PCR) and Western blot analysis.
RESULT: Delta Ct in hypothyroid group on day 7, 14, 21 was 18.89+/-0.92, 18.83+/-0.99, 16.48+/-0.29, separately. While in control group, Delta Ct was 20.32+/-0.92, 20.07+/-0.86, 17.96+/-0.50, separately. Meanwhile, the protein level was detected only in hypothyroid group of 59+/-5% on day 14. On day 21, iNOS protein expression showed a decrease (p<0.05) in hypothyroid group (37+/-3%), compared with control group (58+/-4%).
CONCLUSION: Our findings show the successive changes of the iNOS mRNA and protein levels in early postnatal days of hypothyroid rat brain, and confirm the cross-talk between the TH and NO signaling pathway in developing cerebral cortex of rats.

• 出版日期2010
• 单位浙江大学