A Natural Tetrahydropyrimidine, Ectoine, Ameliorates Ischemia Reperfusion Injury after Intestinal Transplantation in Rats

作者:Pech Thomas; Ohsawa Ichiro; Praktiknjo Michael; Overhaus Marcus; Wehner Sven; von Websky Martin; Abu Elmagd Kareem; van Echten Deckert Gerhild; Kalff Joerg C; Schaefer Nico*
来源:Pathobiology, 2013, 80(2): 102-110.
DOI:10.1159/000342792

摘要

Background/Aims: Ischemia reperfusion (I/R) injury after small bowel transplantation leads to inflammatory reactions and loss of structural integrity with subsequent graft contractile dysfunction in the early postoperative phase. The natural tetrahydropyrimidine ectoine (1-, 4-, 5-, 6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid; THP) protects the ileal mucosa and muscularis against effects of I/R injury in an experimental model of isolated graft reperfusion. The effects of THP treatment were evaluated in an established experimental intestinal transplant model. Methods: Isogenic, orthotopic small bowel transplantation was performed in Lewis rats (6 h cold ischemia time). Perioperative THP treatment (intraluminal/intravascular) groups were compared to vehicle-treated animals (after 3 and 24 h) and non-transplanted controls (n = 5/group). Park%26apos;s score defined the effects of I/R injury. The infiltration of neutrophils, monocytes and macrophages, mRNA expression of IL-6 and TNF-alpha, serum levels of IL-6 and NO and smooth muscle contractility were evaluated. Results: Improved graft outcome after intraluminal and intravascular THP treatment was defined by considerably ameliorated neutrophil infiltration and less histological signs of I/R injury (p %26lt;= 0.05). In the presence of THP, mRNA expression of IL-6 and TNF-alpha and IL-6 and NO serum levels were reduced and smooth muscle function was improved. Conclusion: THP treatment offers protection against the effects of I/R injury in intestinal transplantation in vivo, however, only as supplementary treatment option.

  • 出版日期2013