A logistic model for the detection of circulating tumour cells in human metastatic colorectal cancer

作者：Barbazan Jorge; Vieito Maria; Abalo Alicia; Alonso Alconada Lorena; Muinelo Romay Laura; Alonso Nocelo Marta; Leon Luis; Candamio Sonia; Gallardo Elena; Anido Urbano; Doll Andreas; de los Angeles Casares Maria; Gomez Tato Antonio; Abal Miguel^*; Lopez Lopez Rafael

来源：Journal of Cellular and Molecular Medicine, 2012, 16(10): 2342-2349.

DOI：10.1111/j.1582-4934.2012.01544.x

• metastatic colorectal cancer
• circulating tumour cells
• biomarkers
• GAPDH
• VIL1
• CD45
• logistic model

The accuracy in the diagnosis of metastatic colorectal cancer (mCRC) represents one of the challenges in the clinical management of patients. The detection of circulating tumour cells (CTC) is becoming a promising alternative to current detection techniques, as it focuses on one of the players of the metastatic disease and it should provide with more specific and sensitive detection rates. Here, we describe an improved method of detection of CTC from mCRC patients by combining immune-enrichment, optimal purification of RNA from very low cell numbers, and the selection of accurate PCR probes. As a result, we obtained a logistic model that combines GAPDH and VIL1 normalized to CD45 rendering powerful results in the detection of CTC from mCRC patients (AUROC value 0.8599). We further demonstrated the utility of this model at the clinical setting, as a reliable prognosis tool to determine progression-free survival in mCRC patients. Overall, we developed a strategy that ameliorates the specificity and sensitivity in the detection of CTC, resulting in a robust and promising logistic model for the clinical management of metastatic colorectal cancer patients.

• 出版日期2012-10