Analysis of B-Class Genes NAP3L3 and NAP3L4 in Narcissus tazetta var. chinensis

作者：Li, Xiao Fang^*; Xu, Jing; Yang, Ru; Jia, Lin Yan; Deng, Xin Jie; Xiong, Li Jun; Zhang, Xue Ping; Fang, Qi; Zhang, Wei; Sun, Yue; Xu, Ling

来源：Plant Molecular Biology Reporter, 2013, 31(2): 255-263.

DOI：10.1007/s11105-012-0493-8

• Narcissus tazetta var. chinensis
• Flower organ development
• B-class gene
• NAP3L3
• NAP3L4
• ABC model

Very few flower organ identity genes have been characterized in Chinese narcissus (Narcissus tazetta var. chinensis), which has petaloid sepals. Here, we report the cloning of two full-length B-class genes, namely NAP3L3 and NAP3L4, that are orthologs of the DEFICIENS lineage. Both genes are highly expressed in the second whorl of the perianth and in the stamens. NAP3L4 is also expressed strongly in the ovule. The functions of these two genes were further analyzed using transgenic plants. Ectopic expression of either gene in Arabidopsis gave no obvious floral organ transformation phenotypes. In yeast two-hybrid assays, NAP3L3 and NAP3L4 failed to homodimerize and interacted weakly with each other. The data suggest that these two genes might not be involved in the formation of petaloid sepals. Isolation and functional analysis of other B-class paralogs should be conducted to fully understand petaloid tepal development in Chinese narcissus.

• 出版日期2013-4
• 单位华东师范大学