A Phosphate Transporter Promoter from Arabidopsis thaliana AtPHT1;4 Gene Drives Preferential Gene Expression in Transgenic Maize Roots Under Phosphorus Starvation

作者:Coelho Gracielle T C P; Carneiro Newton P; Karthikeyan Athikkattuvalasu S; Raghothama Kaschandra G; Schaffert Robert E; Brandao Rosangela L; Paiva Luciano V; Souza Isabel R P; Alves Vera M; Imolesi Anderson; Carvalho Carlos H S; Carneiro Andrea A*
来源:Plant Molecular Biology Reporter, 2010, 28(4): 717-723.
DOI:10.1007/s11105-010-0199-8

摘要

Phosphorus (P) stress responsive genes have been identified and characterized, including the high-affinity phosphate transporter AtPHT1;4 from Arabidopsis thaliana. This gene encodes a membrane protein that is primarily expressed in roots under phosphorus deficiency. A 2.3-kb promoter region from AtPHT1;4 has been fused with the beta-glucuronidase (GUS) encoding gene and introduced into maize via biolistic bombardment to evaluate its spatiotemporal activity in a heterologous system. AtPHT1;4::GUS expression is detected preferentially in transgenic maize roots under P deficiency. Further analysis of transgenic plants has also revealed that GUS activity is higher in roots than in leaves by about sixfold. These results demonstrate the ability of AtPHT1;4 promoter to direct expression of the reporter gene in a monocot root system under P stress. This property of AtPHT1;4 promoter makes it useful to engineer maize plants to modify the soil's rhizosphere and increase efficiency of P acquisition under P stress conditions.

  • 出版日期2010-12