Decreased interleukin(IL)-35 Expression is Associated with Active Intraocular Inflammation in Vogt-Koyanagi-Harada (VKH) Disease

作者：Hu, Jianping; Qin, Yirong; Yi, Shenglan; Wang, Chaokui; Yang, Jinglu; Yang, Lu; Wang, Luoziyi; Kijlstra, Aize; Yang, Peizeng^*; Li, Hong^*

来源：Ocular Immunology and Inflammation, 2019, 27(4): 595-601.

DOI：10.1080/09273948.2018.1433306

• IL-35
• VKH disease
• DCs
• uceitis

Purpose: Recent studies have reported that IL-35 has a protective effect in autoimmune disease. In this study, we explored the role of IL-35 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Methods: The IL-35/EBI3 and IL-35/P35 mRNA level was assayed by Real-Time PCR. The level of IL-35 in serum was detected by ELISA. PBMCs and monocyte-derived DCs were cultured with or without IL-35 and the concentration of IL-17, IL-10, IFN-gamma, IL-6, TNF-alpha, and IL-1 beta in supernatants was tested by ELISA. Results: The serum level of IL-35 is reduced in active VKH patients. The mRNA expression of the two subunits IL-35/EBI3 and IL-35/P35 in PBMCs from patients with active VKH was also decreased. IL-35 significantly inhibited IFN-gamma and IL-17 expression and induced IL-10 production by PBMCs and inhibited IL-6 production by monocyte-derived DCs. Conclusion: The current study suggests that a decreased IL-35 expression may be involved in the pathogenesis of VKH disease.

• 出版日期2019-5-19
• 单位重庆医科大学; 兰州大学