Toll-like receptor 2 induces mucosal homing receptor expression and IgA production by human B cells

作者：Liang YanMei; Hasturk Hatice; Elliot Jennifer; Noronha Ansu; Liu Xiuping; Wetzler Lee M; Massari Paola; Kantarci Alpdogan; Winter Harland S; Farraye Francis A; Ganley Leal Lisa M^*

来源：Clinical Immunology, 2011, 138(1): 33-40.

DOI：10.1016/j.clim.2010.09.003

• Adjuvants
• B cells
• Toll-like receptor 2
• Human
• Mucosal immunity

There is a need for developing vaccines that elicit mucosal immunity. Although oral or nasal vaccination methods would be ideal, current strategies have yielded mixed success. Toll-like receptor 2 (TLR2) ligands are effective adjuvants and are currently used in the Hoemophilus influenzae type B vaccine. Induction of humoral immunity in the mucosa is critical for effective vaccination; thus, we sought to determine the effects of TLR2 ligands on human mucosal B cell differentiation. We demonstrate that TLR2 ligands induce CCR9 and CCR10 expression by circulating B cells and increased chemotaxis to cognate chemokines CCL25 and CCL28 suggesting that TLR2 induces B cell homing to the gastrointestinal tract. TLR2 stimulation of B cells also induced J chain and IgA production demonstrating the induction of mucosal-like antibody secreting cells. These observations suggest that vaccines containing TLR2-ligands as adjuvants could induce mucosal B cell immunity even when delivered in a non-mucosal manner.

• 出版日期2011-1