The use of IgG antibodies in conventional and non-conventional immunodiagnostic tests for early prognosis after treatment of Chagas disease

作者：Barbosa Wendling Ana Paula; Vitelli Avelar Danielle Marquete; Sathler Avelar Renato; Geiger Stefan Michael; Teixeira Carvalho Andrea; Gontijo Eliane Dias; Eloi Santos Silvana Maria; Martins Filho Olindo Assis

来源：Journal of Immunological Methods, 2011, 370(1-2): 24-34.

DOI：10.1016/j.jim.2011.05.003

• Chagas disease
• Trypanosoma cruzi
• Treatment
• Cure assessment
• Serology
• Flow cytometry

Treatment success of chronically infected Chagas disease patients is laborious and a positive prognosis often is made only after repetitive serological and/or parasitological examinations with continuous negative results. Recently, we have developed a non-conventional flow-cytometric method in order to detect immunoglobulin G antibodies against live trypomastigote forms of Trypanosoma cruzi and showed its usefulness in the prognosis of treatment success. In the present study, we investigated the performance of flow-cytometric anti-live trypomastigote IgG antibodies (FC-ALTA) and flow-cytometric anti-fixed epimastigote IgG antibodies (FC-AFEA), as well as conventional serological methods, for early monitoring of benznidazole treated Chagas disease patients, e.g. 5 years after treatment The analysis of individual FC-ALTA reactivity along the titration curve before and after treatment, we were able to show, that between 4% and 13% of treated patients under evaluation presented with reduced serological reactivity and segregated from the other patient groups. Similar results were obtained with semi-quantitative, conventional indirect hemagglutination or indirect immunofluorescence. Our data therefore suggest that the combined use of conventional and non-conventional serological methods could provide more suitable cure criteria in early post-therapeutic prognosis of Chagas disease.

• 出版日期2011-7-29