Detection and quantification of Dehalogenimonas and " Dehalococcoides" populations via PCR-based protocols targeting 16S rRNA genes

作者：Yan Jun; Rash Brian A; Rainey Fred A; Moe William M^*

来源：Applied and Environmental Microbiology, 2009, 75(23): 7560-7564.

DOI：10.1128/AEM.01938-09

• 16S rRNA gene
• 16S rRNA gene sequence
• Dehalococcoides
• Louisiana
• Specific primers
• Superfund sites

Members of the haloalkane dechlorinating genus Dehalogenimonas are distantly related to "Dehalococcoides" but share high homology in some variable regions of their 16S rRNA gene sequences. In this study, primers and PCR protocols intended to uniquely target Dehalococcoides were reevaluated, and primers and PCR protocols intended to uniquely target Dehalogenimonas were developed and tested. Use of the genus-specific primers revealed the presence of both bacterial groups in groundwater at a Louisiana Superfund site.

• 出版日期2009