In vitro differentiation of bovine bone marrow-derived mesenchymal stem cells into male germ cells by exposure to exogenous bioactive factors

作者：Cortez J; Bahamonde J; De los Reyes M; Palomino J; Torres C G; Peralta O A

来源：Reproduction in Domestic Animals, 2018, 53(3): 700-709.

DOI：10.1111/rda.13160

<jats:title>Contents</jats:title><jats:p>Mesenchymal stem cells (<jats:styled-content style="fixed-case">MSC</jats:styled-content>) are multipotent progenitor cells defined by their ability to self‐renew and give rise to differentiated progeny. Previous studies have reported that <jats:styled-content style="fixed-case">MSC</jats:styled-content> may be induced in vitro to develop into different types of specialized cells including male gametes. In vitro gamete derivation technology has potential applications as an alternative method for dissemination of elite animal genetics, production of transgenic animals and conservation of endangered species. This study aimed at investigating the in vitro effect of <jats:styled-content style="fixed-case">BMP</jats:styled-content>4, <jats:styled-content style="fixed-case">TGF</jats:styled-content>β1 and <jats:styled-content style="fixed-case">RA</jats:styled-content> on the potential for germ cell (<jats:styled-content style="fixed-case">GC</jats:styled-content>) differentiation of bovine foetal <jats:styled-content style="fixed-case">MSC</jats:styled-content> (bf<jats:styled-content style="fixed-case">MSC</jats:styled-content>) derived from bone marrow (<jats:styled-content style="fixed-case">BM</jats:styled-content>). The effect of <jats:styled-content style="fixed-case">BMP</jats:styled-content>4, <jats:styled-content style="fixed-case">TGF</jats:styled-content>β1 and <jats:styled-content style="fixed-case">RA</jats:styled-content> was analysed on the expression of pluripotent, <jats:styled-content style="fixed-case">GC</jats:styled-content> and male <jats:styled-content style="fixed-case">GC</jats:styled-content> markers on bf<jats:styled-content style="fixed-case">MSC</jats:styled-content> during a 21‐day culture period. bf<jats:styled-content style="fixed-case">MSC</jats:styled-content> cultured under in vitro conditions expressed <jats:styled-content style="fixed-case">OCT</jats:styled-content>4, <jats:styled-content style="fixed-case">NANOG</jats:styled-content> and <jats:styled-content style="fixed-case">DAZL</jats:styled-content>, but lacked expression of <jats:styled-content style="fixed-case">mRNA</jats:styled-content> of <jats:styled-content style="fixed-case">VASA</jats:styled-content>,<jats:styled-content style="fixed-case"> STELLA</jats:styled-content>,<jats:styled-content style="fixed-case"> FRAGILIS</jats:styled-content>,<jats:styled-content style="fixed-case"> STRA</jats:styled-content>8 and <jats:styled-content style="fixed-case">PIWIL</jats:styled-content>2. Treatment with exogenous <jats:styled-content style="fixed-case">BMP</jats:styled-content>4 and <jats:styled-content style="fixed-case">TGF</jats:styled-content>β1 induced a transient increase (<jats:italic>p</jats:italic> &lt; .05) in <jats:styled-content style="fixed-case">DAZL</jats:styled-content> and <jats:styled-content style="fixed-case">NANOG mRNA</jats:styled-content> levels, respectively. However, exposure to <jats:styled-content style="fixed-case">RA</jats:styled-content> was more effective in increasing (<jats:italic>p</jats:italic> &lt; .05) expression of <jats:styled-content style="fixed-case">DAZL</jats:styled-content> and regulating expression of <jats:styled-content style="fixed-case">OCT</jats:styled-content>4 and <jats:styled-content style="fixed-case">mRNA</jats:styled-content> levels of <jats:styled-content style="fixed-case">NANOG</jats:styled-content>. These data suggest that bf<jats:styled-content style="fixed-case">MSC</jats:styled-content> may possess potential for early <jats:styled-content style="fixed-case">GC</jats:styled-content> differentiation, where <jats:styled-content style="fixed-case">OCT</jats:styled-content>4, <jats:styled-content style="fixed-case">NANOG</jats:styled-content> and specially <jats:styled-content style="fixed-case">DAZL</jats:styled-content> may play significant roles in controlling progression along the <jats:styled-content style="fixed-case">GC</jats:styled-content> lineage.</jats:p>

• 出版日期2018-6