Aberrant alternative splicing and extracellular matrix gene expression in mouse models of myotonic dystrophy

作者：Du Hongqing; Cline Melissa S; O**orne Robert J; Tuttle Daniel L; Clark Tyson A; Donohue John Paul; Hall Megan P; Shiue Lily; Swanson Maurice S; Thornton Charles A; Ares Manuel Jr^*

来源：Nature Structural & Molecular Biology, 2010, 17(2): 187-U8.

DOI：10.1038/nsmb.1720

The common form of myotonic dystrophy (DM1) is associated with the expression of expanded CTG DNA repeats as RNA (CUG(exp) RNA). To test whether CUGexp RNA creates a global splicing defect, we compared the skeletal muscle of two mouse models of DM1, one expressing a CTG(exp) transgene and another homozygous for a defective muscleblind 1 (Mbnl1) gene. Strong correlation in splicing changes for similar to 100 new Mbnl1-regulated exons indicates that loss of Mbnl1 explains >80% of the splicing pathology due to CUG(exp) RNA. In contrast, only about half of mRNA-level changes can be attributed to loss of Mbnl1, indicating that CUG(exp) RNA has Mbnl1-independent effects, particularly on mRNAs for extracellular matrix proteins. We propose that CUG(exp) RNA causes two separate effects: loss of Mbnl1 disrupting splicing) and loss of another function that disrupts extracellular matrix mRNA regulation, possibly mediated by Mbnl2. These findings reveal unanticipated similarities between DM1 and other muscular dystrophies.

• 出版日期2010-2