Molecular prognosticators of complex karyotype soft tissue sarcoma outcome: a tissue microarray-based study

作者：Lahat G; Tuvin D; Wei C; Wang W L; Pollock R E; Anaya D A; Bekele B N; Corely L; Lazar A J; Pisters P W; Lev D^*

来源：Annals of Oncology, 2010, 21(5): 1112-1120.

DOI：10.1093/annonc/mdp459

• matrix metalloproteinase
• molecular markers
• outcome
• prognostic factors
• soft tissue sarcoma

Materials and methods: A large (n = 205) clinically annotated tissue microarray (TMA) was constructed and immunostained for several tumor-related markers. Staining was scored via an automated Ariol image analysis system; data were statistically analyzed to evaluate the correlation of clinicopathological and molecular variables with overall survival (OS) and local recurrence.
Results: Multivariable analysis identified older age [hazard ratio (HR) 1.62, P < 0.0001], nonextremity location (HR 2.95, P = 0.001), high tumor grade (HR 2.5, P = 0.02), and increased matrix metalloproteinase (MMP) 2 expression (HR 1.74, P = 0.04) as predictors for poor OS. Similarly, older age (HR 1.51, P = 0.008), nonextremity location (HR 4.09, P = 0.001), and increased MMP2 expression (HR 6.28, P = 0.006) were all found to correlate with shorter local recurrence-free interval. High nuclear p53 expression was associated with shorter STS local recurrence-free interval, with a trend toward significance.
Conclusions: Data presented indicate that a clinically annotated TMA can be utilized to identify STS-related prognostic markers. Specifically, MMP2 and nuclear p53 should be further evaluated for their potential inclusion in complex karyotype STS staging systems.

• 出版日期2010-5