A Novel Low-Cost Sampler for Rapid Diagnosis of Infectious Agents in Tissue

作者：Hong Ni Ning^*; Li Tao; Zhang Deng Xiang; Ran Long Zhong; Yan Xiu Gang

来源：Journal of Animal and Veterinary Advances, 2012, 11(24): 4563-4566.

• Sampler
• tissue
• PCR
• molecular diagnosis
• cross-contamination

Preparing biological tissue samples is an extremely tedious and time-consuming laboratory task, albeit one critical for rapid diagnoses of infectious diseases. Here, researchers reported a novel, low-cost sampling technique for rapid and accurate removal of minute tissue portions from various animal organs. Each sampled tissue portion could be easily dispersed and homogenized in storage solution in. 2.0 mL microcentrifuge tube and was sufficient for the molecular diagnosis of the presence or absence of infectious disease using the Polymerase Chain Reaction (PCR) technique. This new sampling technique is beneficial as it provides both uniformity for high-throughput sampling and minimization of cross-contamination.

• 出版日期2012
• 单位贵州医科大学