Attenuation of LPS-induced apoptosis in NGF-differentiated PC12 cells via NF-kappa B pathway and regulation of cellular redox status by an oxazine derivative

作者：Ansari Niloufar; Khodagholi Fariba^*; Amini Mohsen; Shaerzadeh Fatemeh

来源：Biochimie, 2011, 93(5): 899-908.

DOI：10.1016/j.biochi.2011.01.012

• Apoptosis
• LPS
• NF-kappa B
• Oxazine derivative
• PC12 cells

Neuronal cell death due to apoptosis is a common characteristic of neurodegenerative diseases. In this study, we report protective effect of 2-ethoxy-4,5-diphenyl-1,3-oxazine-6-one (EDPOO) against lipopolysaccharide (LPS)-induced cell death in rat pheochromocytoma (PC12) cells, as assessed by MTT test, acridine orange/ethidium bromide staining, determination of Bax, Bcl-2 and caspase-3 levels. We further show that this compound could increase heat shock proteins Hsp-70 and Hsp-32 levels. EDPOO also modulates nuclear levels of Nrf2 and NF-kappa B, transcription factors that are activated by intracellular reactive oxygen species and/or mediators generated due to chemical exposure of cells. Pretreatment of the cells with this oxazine derivative also increases gamma-GCS level, as well as antioxidant enzyme activities, in a dose-dependent manner. Protective effect of this compound could represent a promising approach for treatment of neurodegenerative diseases.

• 出版日期2011-5