DOSE RESPONSE EFFECT OF Paracoccidioides brasiliensis IN AN EXPERIMENTAL MODEL OF ARTHRITIS

作者：Loth Eduardo Alexandre^*; Biazim Samia Khalil; Fermino Ferreira dos Santos Jose Henrique; Puccia Rosana; Brancalhao Rosimeire Costa; Chasco Lucineia de Fatima; Gandra Rinaldo Ferreira; Garcia Simao Rita de Cassia; de Franco Marcello Fabiano

来源：Revista do Instituto de Medicina Tropical de Sao Paulo, 2014, 56(3): 259-264.

DOI：10.1590/S0036-46652014000300012

• Paracoccidioidomycosis
• Paracoccidioides brasiliensis
• Arthritis
• Inflammation

Paracoccidioidomycosis (PCM) is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb) and corresponds to prevalent systemic mycosis in Latin America. The aim of the present work was to evaluate the dose response effect of the fungal yeast phase for the standardization of an experimental model of septic arthritis. The experiments were performed with groups of 14 rats that received doses of 10(3), 10(4) or 10(5) P. brasiliensis (Pb18) cells. The fungi were injected in 50 mu L of phosphate-buffered saline (PBS) directly into the knee joints of the animals. The following parameters were analyzed in this work: the formation of swelling in knees infused with yeast cells and the radiological and anatomopathological alterations, besides antibody titer by ELISA. After 15 days of infection, signs of inflammation were evident. At 45 days, some features of damage and necrosis were observed in the articular cartilage. The systemic dissemination of the fungus was observed in 11% of the inoculated animals, and it was concluded that the experimental model is able to mimic articular PCM in humans and that the dose of 10(5) yeast cells can be used as standard in this model.

• 出版日期2014-6