Asymmetric methyl group labeling as a probe of membrane protein homo-oligomers by NMR spectroscopy

作者：Traaseth Nathaniel J; Verardi Raffaello; Veglia Gianluigi^*

来源：Journal of the American Chemical Society, 2008, 130(8): 2400-2401.

DOI：10.1021/ja711499r

We present an asymmetric isotopic labeling strategy for probing unambiguously membrane protein binding interfaces in homo-oligomers. This technique is highly sensitive, rapid, and requires only one C-13 methyl-edited nuclear Overhauser effect spectroscopy (NOESY) spectrum. As a proof of principle, we apply this technique to the homo-pentameric membrane protein phospholamban, defining the leucine-isoleucine zipper interface between its monomers with atomistic resolution.

• 出版日期2008-2-27