Direct electrochemistry and electrocatalysis of hemoglobin entrapped in composite matrix based on chitosan and CaCO3 nanoparticles

作者：Shan, Dan; Wang, Shanxia; Xue, Huaiguo^*; Cosnier, Serge

来源：Electrochemistry Communications, 2007, 9(4): 529-534.

DOI：10.1016/j.elecom.2006.10.032

• calcium carbonate
• nanoparticles
• chitosan
• hemoglobin
• direct electron transfer

The direct electron transfer between hemoglobin (Hb) and the underlying glassy carbon electrode (GCE) can be readily achieved via a high biocompatible composite system based on biopolymer chitosan (CHT) and inorganic CaCO3 nanoparticles (nano-CaCO3). Cyclic voltammetry of Hb-CHT/nano-CaCO3/GCE showed a pair of stable and quasi-reversible peaks for HbFe(III)/Fe(II) redox couple in pH 7.0 buffer. The electrochemical reaction of Hb immobilized in CHT/nano-CaCO3 composite matrix exhibited a surface-controlled process accompanied by electron and proton transfer. The electron transfer rate constant was estimated to be 1.8 s(-1). This modified electrode showed a high thermal stability up to 60 degrees C. The apparent Michaelis-Menten constant was calculated to be 7.5 X 10(-4) M, indicating a high catalytic activity of the immobilized Hb toward H2O2. The interaction between Hb and this nano-hybrid material was also investigated using FT-IR and UV-vis spectroscopy, indicating that Hb retained its native structure in this hybrid matrix.

• 出版日期2007-4
• 单位扬州大学