In vitro plant regeneration through direct organogenesis in Populus deltoides clone G48 from petiole explants

作者：Thakur Ajay Kumar^*; Saraswat A; Srivastava D K

来源：Journal of Plant Biochemistry and Biotechnology, 2012, 21(1): 23-29.

DOI：10.1007/s13562-011-0067-0

• Direct organogenesis
• Populus deltoides
• Petiole explants
• Regeneration

A rapid and efficient protocol is developed for in vitro plantlet regeneration of Populus deltoides clone G48 using petiole explants. The highest frequency of shoot regeneration (74.75%) from petiole was obtained on MS medium supplemented with 0.50 mg/l BAP and 0.20 mg/l IAA. The regenerated shoots started turning brown and necrotic after 10-15 days in culture. To overcome the browning problem, the explants along with the developing shoot buds were transferred to modified MS medium containing 0.50 mg/l BAP, 0.20 mg/l IAA, 15 mg/l AdS, 0.1% PVP, 100 mg/l casein hydrolysate, 50 mg/l L-glutamine, 250 mg/l (NH(4))(2)SO(4) and 0.5% agar. Shoot multiplication and elongation took place on the same medium. Indole-3-acetic acid at 0.10 mg/l was most effective for root regeneration. Using the current protocol, it took 2 months to regenerate plantlets. The in vitro regenerated plantlets were successfully acclimatized and established in greenhouse conditions. This regeneration system using petiole explants provides a foundation for Agrobacterium-mediated genetic transformation of P. deltoides clone G48 for incorporation of various silviculturally important traits.

• 出版日期2012-1