科研之友
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摘要
The glycosaminoglycans (GAGs) found in the mammalian oviductal tissue and luminal secretory fluid influence the fertilization processes in various patterns such as sperm storage, viability and capacitation. The aim of this study was to confirm the appearance of hyaluronan and syndecan-1 in the swamp buffalo oviduct consisting of uterotubal junction (UTJ), isthmus, ampulla and infundibulum during oestrous cycle using histochemical and immunohistochemical techniques. The non-sulphated GAGs, i.e. hyaluronan (HA), were noticeably localized in the subepithelial connective tissue layer or lamina propria of all segments while the epithelial HA-positive staining was demonstrated only in the UTJ and isthmus (sperm reservoir) at the follicular phase. In contrast, the example of the sulphated GAGs, i.e. synclecan-1, were seen along the epithelial lining of all portions and both oestrous stages, however, the syndecan-1 positive labeled on the epithelial lining surface was intensely observed only in the UTJ and isthmus. Therefore, the present results established for the first time that non-sulphated and sulphated GAGs certainly contained in the swamp buffalo oviductal tissue. The noticeable localization of HA in the UTJ and isthmus might indicate the regulation of HA in the forming of sperm reservoir to maintain viability of spermatozoa before ovulation whereas the expression of syndecan-1 may play a vital role in the regulation of sperm adhesion and the initiation of sperm capacitation.
- 出版日期2011-9
