Developing a genetically encoded green fluorescent protein mutant for sensitive light-up fluorescent sensing and cellular imaging of Hg(II)

作者：Jiang Tao; Guo Daiping; Wang Qian; Wu Xin; Li Zhao; Zheng Zhenhua; Yin Boyuan; Xia Lin; Tang Jixian; Luo Wenxin; Xia Ningshao; Jiang Yunbao^*

来源：Analytica Chimica Acta, 2015, 876: 77-82.

DOI：10.1016/j.aca.2015.03.026

• Green fluorescent protein-based sensor
• Mercury ion
• Fluorescence light-up
• Cellular imaging

Hg(II) is well-known for quenching fluorescence in a distance dependent manner. Nevertheless, when we exposed the fluorophore of a green fluorescent protein (GFP) toward Hg(II), through H148C mutation, the GFP fluorescence could be "lighted up" by Hg(II) down to sub-nM level. The detection linear range is 0.5-3.0 nM for protein solutions at 8.0 nM. The GFPH148C protein displayed a promising selectivity toward Hg(II) and also the cellular imaging capacity. Spectra measurements suggested that the ground-state redistribution of protein contributed to the fluorescence enhancement, which was found not limited to Hg(II), and thus presented an opening for building a pool of GFP-based chemosensors toward other heavy metal ions.

• 出版日期2015-5-30
• 单位厦门大学