Asiatic Acid Exhibits Anti-inflammatory and Antioxidant Activities aganist Lipopolysaccharide and D-Galactosamine-Induced Fulminant Hepatic Failure

作者：Lv, Hongming; Qi, Zhimin; Wang, Sisi; Feng, Haihua; Deng, Xuming; Ci, Xinxin^*

来源：Frontiers in Immunology, 2017, 8: 785.

DOI：10.3389/fimmu.2017.00785

• asiatic acid
• inflammation
• oxidative stress
• fulminant hepatic failure
• AMPK/Nrf2
• PDCD4

Inflammation and oxidative stress are essential for the pathogenesis of fulminant hepatic failure (FHF). Asiatic acid (AA), which is a pentacyclic triterpene that widely occurs in various vegetables and fruits, has been reported to possess antioxidant and anti-inflammatory properties. In this study, we investigated the protective effects of AA against lipopolysaccharide (LPS) and D-galactosamine (GalN)-induced FHF and the underlying molecular mechanisms. Our findings suggested that AA treatment effectively protected against LPS/D-GalN-induced FHF by lessening the lethality; decreasing the alanine transaminase and aspartate aminotransferase levels, interleukin (IL)-1 beta, IL-6, and tumor necrosis factor-alpha production, malondialdehyde formation, myeloperoxidase level and reactive oxygen species generation (i.e., H2O2, NO, and O-2(-)), and increasing the glutathione and superoxide dismutase contents. Moreover, AA treatment significantly inhibited mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappa B) signaling pathway activation via the partial induction of programmed cell death 4 (PDCD4) protein expressions, which are involved in inflammatory responses. Furthermore, AA treatment dramatically induced the expression of the glutamate-cysteine ligase modifier subunit, the glutamate-cysteine ligase catalytic subunit, heme oxygenase-1, and NAD (P) H: quinoneoxidoreductase 1 (NQO1), which are largely dependent on activation of the nuclear factor-erythroid 2-related factor 2 (Nrf2) through the induction of AMP-activated protein kinase (AMPK) and glycogen synthase kinase-3 beta (GSK3 beta) phosphorylation. Accordingly, AA exhibited protective roles against LPS/D-GalN-induced FHF by inhibiting oxidative stress and inflammation. The underlying mechanism may be associated with the inhibition of MAPK and NF-kappa B activation via the partial induction of PDCD4 and upregulation of Nrf2 in an AMPK/GSK3 beta pathway activation-dependent manner.

• 出版日期2017-7-7
• 单位吉林大学