Neuroprotective iridoid glycosides from Cornus officinalis fruits against glutamate-induced toxicity in HT22 hippocampal cells

作者：Jeong Eun Ju; Kim Tae Bum; Yang Heejung; Kang So Young; Kim Sun Yeou; Sung Sang Hyun; Kim Young Choong^*

来源：Phytomedicine, 2012, 19(3-4): 317-321.

DOI：10.1016/j.phymed.2011.08.068

• Cornus officinalis
• Iridoid glycoside
• 7-O-Butylmorroniside
• HT22
• Neuroprotective

The methanolic extract of the fruits of Comas officinalis S et Z. (Cornaceae) showed the significant neuroprotective activity against glutamate-induced toxicity in HT22 hippocampal cells. Chemical profile of n-BuOH fraction of the methanolic extract of C. officinalis fruits, which showed the most potent activity, was established using HPLC-diode array detector-electrospray-MS (HPLC-DAD-ESI-MS). Through bioactivity-guided isolation, five iridoid glycosides including one new compound, 7-O-butylmorroniside (1), loganin (2), morroniside (3), 7R-O-methylmorroniside (4), 7S-O-methylmorroniside (5) were isolated from the n-BuOH fraction. The protective activities of the isolated compounds, themselves, were not statistically significant. However, the hydrolyzed products of compounds 1,4 and 5 significantly protected glutamate-injured HT22 cells up to 78 +/- 2.2%, 60 +/- 3.2% and 59 +/- 2.5% of non-treated control, respectively.

• 出版日期2012-2-15