Anabolic action of parathyroid hormone regulated by the beta(2)-adrenergic receptor

作者：Hanyu Ryo; Wehbi Vanessa L; Hayata Tadayoshi; Moriya Shuichi; Feinstein Timothy N; Ezura Yoichi; Nagao Masashi; Saita Yoshitomo; Hemmi Hiroaki; Notomi Takuya; Nakamoto Tetsuya; Schipani Ernestina; Takeda Shu; Kaneko Kazuo; Kurosawa Hisashi; Karsenty Gerard; Kronenberg Henry M; Vilardaga Jean Pierre^*; Noda Masaki

来源：Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(19): 7433-7438.

DOI：10.1073/pnas.1109036109

• bone anabolism
• bone cells

Parathyroid hormone (PTH), the major calcium-regulating hormone, and norepinephrine (NE), the principal neurotransmitter of sympathetic nerves, regulate bone remodeling by activating distinct cell-surface G protein-coupled receptors in osteoblasts: the parathyroid hormone type 1 receptor (PTHR) and the beta(2)-adrenergic receptor (beta(2)AR), respectively. These receptors activate a common cAMP/PKA signal transduction pathway mediated through the stimulatory heterotrimeric G protein. Activation of beta(2)AR via the sympathetic nervous system decreases bone formation and increases bone resorption. Conversely, daily injection of PTH (1-34), a regimen known as intermittent (i) PTH treatment, increases bone mass through the stimulation of trabecular and cortical bone formation and decreases fracture incidences in severe cases of osteoporosis. Here, we show that iPTH has no osteoanabolic activity in mice lacking the beta(2)AR. beta(2)AR deficiency suppressed both iPTH-induced increase in bone formation and resorption. We showed that the lack of beta(2)AR blocks expression of iPTH-target genes involved in bone formation and resorption that are regulated by the cAMP/PKA pathway. These data implicate an unexpected functional interaction between PTHR and beta(2)AR, two G protein-coupled receptors from distinct families, which control bone formation and PTH anabolism.

• 出版日期2012-5-8