Phosphoproteonne Analysis of Streptomyces Development Reveals Extensive Protein Phosphorylation Accompanying Bacterial Differentiation

作者：Manteca Angel^*; Ye Juanying; Sanchez Jesus; Jensen Ole Nerregaard

来源：Journal of Proteome Research, 2011, 10(12): 5481-5492.

DOI：10.1021/pr200762y

• Streptomyces
• phosphoproteomics
• differentiation
• sporulation
• LC-MS/MS

Streptomycetes are bacterial species that undergo a complex developmental cycle that includes programmed cell death (PCD) events and sporulation. They are widely used in biotechnology because they produce most clinically relevant secondary metabolites. Although Streptomyces coelicolor is one of the bacteria encoding the largest number of eukaryotic type kinases, the biological role of protein phosphorylation in this bacterium has not been extensively studied before. In this issue, the variations of the phosphoproteome of S. coelicolor were characterized. Most distinct Ser/Thr/Tyr phosphorylation events were detected during the presporulation and sporulation stages (80%). Most of these phosphorylations were not reported before in Streptomyces, and included sporulation factors, transcriptional regulators, protein kinases and other regulatory proteins. Several of the identified phosphorylated proteins, FtsZ, DivIVA, and FtsH2, were previously demonstrated to be involved in the sporulation process. We thus established for the first time the widespread occurrence and dynamic features of Ser/Thr/Tyr protein phosphorylation in a bacteria species and also revealed a previously unrecognized phosphorylation motif "x(pT)xEx".

• 出版日期2011-12