Optimisation of simultaneous saccharification and fermentation of wheat straw for ethanol production

作者：Zhang, Wei; Lin, Yan^*; Zhang, Qi; Wang, Xinze; Wu, Deyi; Kong, Hainan

来源：Fuel, 2013, 112: 331-337.

DOI：10.1016/j.fuel.2013.05.064

• Ethanol yield
• Box-Behnken design (BBD)
• Response surface methodology (RSM)
• Simultaneous saccharification and fermentation (SSF)
• Wheat straw (WS)

With the goal of obtaining a high ethanol yield, wheat straw (WS) was pretreated with a 1.0% (w/w) NaOH solution. Further parameters, including temperature, enzyme loading, yeast concentration and pH that would affect the ethanol yield through simultaneous saccharification and fermentation (SSF), were optimised by means of Box-Behnken design (BBD), a response surface methodology (RSM). All of the experiments were carried out with an initial solid content of 16.0% (w/v) and fermentation time of 120 h. A well fitted regression equation with an R-2 value of 0.9603 was obtained. The predicted maximum response value (ethanol yield) was 69.49% under the optimised conditions for SSF: temperature of 37.5 degrees C, enzyme loading of 30 FPU/g substrate, yeast concentration of 10 g/L and pH of 4.6. The validation experimental result showed that the maximum ethanol yield was 70.76%, which was in accordance with the predicted value.

• 出版日期2013-10
• 单位上海交通大学