A simple and sensitive fluorometric dopamine assay based on silica-coated CdTe quantum dots

作者：Wang, Bei; Chen, Miao-miao; Zhang, Hai-qing; Wen, Wei; Zhang, Xiu-hua^*; Wang, Sheng-fu

来源：Microchimica Acta, 2017, 184(9): 3189-3196.

DOI：10.1007/s00604-017-2270-8

• Nanoparticles
• Surface modification
• Tyrosinase
• Fluorescence quenching
• Electron transfer processes

A fluorescence assay is described for the fluorometric determination of dopamine (DA). It based on the use of silica-coated CdTe quantum dots (QD@SiO2). These were fabricated through a hydrothermal process. When DA is added to a solution of the QD@SiO2 and then oxidized by oxygen under catalytic action of tyrosinase to form dopamine quinone, the fluorescence of QD@SiO2 (acquired at excitation/emission wavelengths of 310/525 nm) decreases due to an electron transfer quenching processes. The assay has a linear calibration plot in the 0.05 to 30 mu M DA concentration range and a 12.5 nM detection limit (at an S/N ratio of 3). The method was applied to the determination of DA in spiked human serum samples.

• 出版日期2017-9
• 单位湖北大学