Draft genome sequence of Bacillus sp M13(2017), a multidrug-resistant subclass B1 blaNDM-producing, spore-forming bacterium isolated from China

作者：Liu, Tingting; Zhu, Liying^*; Zhang, Zhidong; Jiang, Ling^*; Huang, He

来源：Journal of Global Antimicrobial Resistance, 2018, 14: 152-153.

DOI：10.1016/j.jgar.2018.07.002

• Bacillus sp.
• Infection
• Whole-genome sequencing
• WGS
• Multidrug resistance

Objectives: Bacillus spp., which have relevance to pseudo-epidemics in clinics, may cause serious infections as well as high morbidity and mortality in hospitalised patients with haematological malignancies. Here we report the isolation of Bacillus sp. M13(2017) with a multidrug-resistant phenotype and spore-producing structures, a new strain isolated from radiation-polluted soil in Xinjiang Uyghur Autonomous Region, China. @@@ Methods: The draft genome of Bacillus sp. M13(2017) was obtained using an Illumina HiSeq(TM) 2000 next-generation DNA sequencing platform. Subsequently, clean sequencing data were assembled using SOAPdenovo and were optimised using GapCloser. @@@ Results: The draft genome sequence of Bacillus sp. M13(2017) includes 5 873 661 bp, a GC content of 34.84% and is assembled into 6108 open reading frames, of which ca. 5.08% and 1.76% are related to spore formation and antimicrobial resistance, respectively. The bla(NDM) gene encoding a subclass B1 metallo-beta-lactamase was also discovered in this isolate. @@@ Conclusions: The genome sequence of Bacillus sp. M13(2017) reported here will be a reference for comparative analysis with genomic and clinically important multidrug resistance features of Bacillus sp.

• 出版日期2018-9
• 单位南京工业大学; 新疆农业科学院