Hierarchical organization of the plasma membrane: Investigations by single-molecule tracking vs. fluorescence correlation spectroscopy

作者：Kusumi Akihiro^*; Shirai Yuki M; Koyama Honda Ikuko; Suzuki Kenichi G N; Fujiwara Takahiro K

来源：FEBS LETTERS, 2010, 584(9): 1814-1823.

DOI：10.1016/j.febslet.2010.02.047

• Plasma membrane
• Single-molecule tracking
• Fluorescence correlation spectroscopy
• Raft domains
• Compartmentalization
• Actin filament

Single-molecule tracking and fluorescence correlation spectroscopy (FCS) applied to the plasma membrane in living cells have allowed a number of unprecedented observations, thus fostering a new basic understanding of molecular diffusion, interaction, and signal transduction in the plasma membrane. It is becoming clear that the plasma membrane is a heterogeneous entity, containing diverse structures on nano-meso-scales (2-200 nm) with a variety of lifetimes, where certain membrane molecules stay together for limited durations. Molecular interactions occur in the time-dependent inhomogeneous two-dimensional liquid of the plasma membrane, which might be a key for plasma membrane functions.

• 出版日期2010-5-3