Abnormal glycogen chain length pattern, not hyperphosphorylation, is critical in Lafora disease

作者：Nitschke Felix; Sullivan Mitchell A; Wang Peixiang; Zhao Xiaochu; Chown Erin E; Perri Ami M; Israelian Lori; Juana Lopez Lucia; Bovolenta Paola; Rodriguez de Cordoba Santiago; Steup Martin; Minassian Berge A^*

来源：EMBO Molecular Medicine, 2017, 9(7): 906-917.

DOI：10.15252/emmm.201707608

• glycogen chain length
• glycogen phosphorylation
• Lafora disease
• laforin
• malin

Lafora disease (LD) is a fatal progressive epilepsy essentially caused by loss-of-function mutations in the glycogen phosphatase laforin or the ubiquitin E3 ligase malin. Glycogen in LD is hyperphosphorylated and poorly hydrosoluble. It precipitates and accumulates into neurotoxic Lafora bodies (LBs). The leading LD hypothesis that hyperphosphorylation causes the insolubility was recently challenged by the observation that phosphatase-inactive laforin rescues the laforin-deficient LD mouse model, apparently through correction of a general autophagy impairment. We were for the first time able to quantify brain glycogen phosphate. We also measured glycogen content and chain lengths, LBs, and autophagy markers in several laforin-or malin-deficient mouse lines expressing phosphatase-inactive laforin. We find that: (i) in laforindeficient mice, phosphatase-inactive laforin corrects glycogen chain lengths, and not hyperphosphorylation, which leads to correction of glycogen amounts and prevention of LBs; (ii) in malin-deficient mice, phosphatase-inactive laforin confers no correction; (iii) general impairment of autophagy is not necessary in LD. We conclude that laforin's principle function is to control glycogen chain lengths, in a malin-dependent fashion, and that loss of this control underlies LD.

• 出版日期2017-7
• 单位河北医科大学