Acute Ethanol Inhibition of Adult Hippocampal Neurogenesis Involves CB1 Cannabinoid Receptor Signaling

作者：Khatri Dal; Laroche Genevieve; Grant Marion L; Jones Victoria M; Vetreno Ryan P; Crews Fulton T; Mukhopadhyay Somnath^*

来源：Alcoholism: Clinical and Experimental Research , 2018, 42(4): 718-726.

DOI：10.1111/acer.13608

• Cannabinoid Receptor
• Ethanol
• Alcohol
• Adult Neurogenesis
• Dentate Gyrus

BackgroundChronic ethanol (EtOH) exposure has been found to inhibit adult hippocampal neurogenesis in multiple models of alcohol addiction. However, acute EtOH inhibition of adult neurogenesis is not well studied. Although many abused drugs have been found to inhibit adult neurogenesis, few have studied cannabinoids or cannabinoids with EtOH, although human use of both together is becoming more common. We used an acute binge alcohol drinking model in combination with select cannabinoid receptor agonists and antagonists to investigate the actions of each alone and together on hippocampal neurogenesis.
MethodsAdult male Wistar rats were treated with an acute binge dose of EtOH (5g/kg, i.g.), cannabinoid 1 receptor (CB1R) or cannabinoid 2 receptor (CB2R) agonists, as well as selective cannabinoid (CB) antagonists, alone or combined. Hippocampal doublecortin (DCX), Ki67, and activated cleaved caspase-3 (CC3) immunohistochemistry were used to assess neurogenesis, neuroprogenitor proliferation, and cell death, respectively.
ResultsWe found that treatment with EtOH or the CB1R agonist, arachidonoyl-2-chloroethylamide (ACEA), and the combination significantly reduced DCX-positive neurons (DCX+IR) in dentate gyrus (DG) and increased CC3. Further, using an inhibitor of endocannabinoid metabolism, for example, JZL195, we also found reduced DCX+IR neurogenesis. Treatment with 2 different CB1R antagonists (AM251 or SR141716) reversed both CB1R agonist and EtOH inhibition of adult neurogenesis. CB2R agonist HU-308 treatment did not produce any significant change in DCX+IR. Interestingly, neither EtOH nor CB1R agonist produced any alteration in cell proliferation in DG as measured by Ki67 + cell population, but CC3-positive cell numbers increased following EtOH or ACEA treatment suggesting an increase in cell death.
ConclusionsTogether, these findings suggest that acute CB1R cannabinoid receptor activation and binge EtOH treatment reduce neurogenesis through mechanisms involving CB1R.

• 出版日期2018-4