DROSOPHILA MELANOGASTER - AN EMBRYONIC MODEL FOR STUDYING BEHAVIORAL AND BIOCHEMICAL EFFECTS OF MANGANESE EXPOSURE

作者：Lausmann Ternes Ana Paula; Zemolin Ana Paula; da Cruz Litiele Cezar; da Silva Gustavo Felipe; Fleig Saidelles Ana Paula; de Paula Mariane Trindade; Wagner Caroline; Golombieski Ronaldo Medeiros; de Moraes Flores Erico Marlon; Picoloto Rochele Sogari; Pereira Antonio Batista; Franco Jeferson Luis; Posser Thais^*

来源：EXCLI Journal, 2014, 13: 1239-1253.

• Manganese
• Drosophila melanogaster
• MAPK
• oxidative stress
• Thioredoxin reductase

Embryonic animals are especially susceptible to metal exposure. Manganese (Mn) is an essential element, but in excess it can induce toxicity. In this study we used Drosophila melanogaster as an embryonic model to investigate biochemical and behavioral alterations due to Mn exposure. Flies were treated with standard medium supplemented with MnCl2 at 0.1 mM, 0.5 mM or 1 mM from the egg to the adult stage. At 0.5 mM and 1 mM Mn, newly ecloded flies showed significantly enhanced locomotor activity when assessed by negative geotaxis behavior. In addition, a significant increase in Mn levels (p < 0.0001) was observed, while Ca, Fe, Cu, Zn and S levels were significantly decreased. A significant drop in cell viability occurred in flies exposed to 1 mM Mn. There was also an induction of reactive oxygen species at 0.5 mM and 1 mM Mn (p < 0.05). At 1 mM, Mn increased Catalase (p < 0.005), Superoxide Dismutase (p < 0.005) and Hsp83 (p < 0.0001) mRNA expression, without altering Catalase or Superoxide Dismutase activity; the activity of Thioredoxin reductase and Glutatione-S-transferase enzymes was increased. Mn treatment did not alter ERK or JNK1/2 phosphorylation, but at 1 mM caused an inhibition of p38(MAPK) phosphorylation. Together these data suggest mechanisms of adaptation in the fly response to Mn exposure in embryonic life.

• 出版日期2014