A multi-biomarker approach to assess the impact of farming systems on black tiger shrimp (Penaeus monodon)

作者:Huynh Thi Tu*; Silvestre Frederic; Wang Neil; Thome Jean Pierre; Nguyen Thanh Phuong; Kestemont Patrick
来源:Chemosphere, 2010, 81(10): 1204-1211.
DOI:10.1016/j.chemosphere.2010.09.039

摘要

This study examined the advantages of the use of biomarkers as an early warning system by applying it to different shrimp farming systems in Soctrang and Camau provinces main shrimp producers in Mekong River Delta Vietnam Shrimp were collected at 15 different farms divided Into four different farming systems three farms were converted from originally rice paddies into intensive shrimp farming systems (IS1 IS2 IS3) three farms were rice-shrimp integrated farming systems (RS4 RS5 RS6) three farms were intensive farming systems (IS7 IS8 IS9) six farms were extensive shrimp farming systems (From ES1 to ES6) Lipid peroxidation (LPO) and total glutathione (GSH) were measured as well as catalase (CAT) glutathione peroxidase (GPX) glutathione S-transferase (GST) and acetylcholinesterase activities (ACHE) Organ specificity was observed between gills and hepatopancreas with generally higher activity of GST in gills (GSTG) whereas the contrary was observed for LPO level in gills (LPOG) Hierarchical clustering and principal component analysis clearly indicated that shrimp reared in extensive culture system formed a distinct group from those reared in intensive or rice-shrimp integrated systems CAT in gills (CATG) GPX in gills (GPXG) and hepatopancreas (GPXHP) and ACHE in muscle (ACHEM) of shrimp collected in extensive farms showed a general higher level than those in intensively farmed shrimp On the contrary we observed clear high levels of GSTG and GST in hepatopancreas (GSTHP) and LPOG and hepatopancreas (LPOHP) of shrimp sampled in intensive and rice-shrimp integrated systems Thus we propose that LPO and CAT GPX GST and ACHE can be used as a set of biomarkers for the assessment of health condition and can discriminate between shrimp cultivated in different farm

  • 出版日期2010-11

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