In vitro propagation of Citrus indica Tanaka-An endangered progenitor species

作者：Laskar M A^*; Hynniewta N; Rao C S

来源：Indian Journal of Biotechnology, 2009, 8(3): 311-316.

• Citrus indica endangered
• ex situ adaptibility
• leaf-derived callus
• progenitor
• shoot organogenesis

A method for in vitro propagation of Citrus indices Tanaka by shoot organogenesis from leaf-derived callus was developed. Regenerative calli were induced on MS medium supplemented with 0.01 mg L(-1) TDZ, and 0.1 mg L(-1) NAA. Shoots were regenerated on WPM medium supplemented with 0.5 mg L(-1) BAP, 0.25 mg L(-1) TDZ and 0.25 mg L(-1) NAA. Regenerated shoots were rooted on MS medium supplemented with 1.0 mg L(-1) NAA. Sixty per cent of the rooted plantlets were acclimatized successfully under ex situ conditions.

• 出版日期2009-7