Determination of serum triglyceride by enzyme electrode using covalently immobilized enzyme on egg shell membrane

作者：Narang Jagriti; Minakshi; Bhambi Manu; Pundir C S^*

来源：International Journal of Biological Macromolecules, 2010, 47(5): 691-695.

DOI：10.1016/j.ijbiomac.2010.09.001

• TG
• Biosensor lipase
• GK
• GPO
• Egg shell membrane

A mixture of commercial lipase glycerol kinase and glycerol-3-phosphate oxidase was co-immobilized onto egg shell membrane through covalent coupling A method is described for fabrication of a triglyceride (TG) biosensor using egg shell membrane bound enzymes The biosensor measured current i e flow of electrons generated from H(2)O(2) maximally when polarized at 0 4 V The biosensor showed optimum response within 10 sec at pH 7 0 and 35 C The current was in proportion to concentration of TG in the range 0 56-2 25 mM An amperometric method was developed for determination of TG employing this enzyme electrode The minimum detection limit of the method was 0 28 mM The analytic recovery of added TG was 95 00% and 96 50% Within batch and between batch coefficients of variations (CV) were <2 14% and <3 48% respectively A good correlation (r = 0 985) was obtained between serum TG level by standard enzymic colorimetric method and the present method Serum substances such as urea uric acid glucose cholesterol ascorbic acid and

• 出版日期2010-12-1