Identification of differentially expressed proteins in SH-SY5Y cells treated with resveratrol

作者：Wang Ying; Dong Zhong; Fan Hongyan; Chang Ming; Li Guoyi; Hu Linsen^*

来源：Neural Regeneration Research, 2011, 6(21): 1612-1617.

DOI：10.3969/j.issn.1673-5374.2011.21.002

• resveratrol
• SH-SY5Y cells
• two-dimensional difference gel electrophoresis
• proteomics
• neural regeneration

To gain insight into the molecular mechanisms of resveratrol-mediated neuroprotection, two-dimensional difference gel electrophoresis in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to identify proteins differentially-expressed in SH-SY5Y cells treated with resveratrol. Compared with the control group, resveratrol treatment significantly affected the expression of four proteins: endoplasmic reticulum oxidoreductin 1-like protein alpha, p21-activated kinase 1, Archain 1, and T cell receptor beta chain. The former three were downregulated and the latter was upregulated. These proteins are primarily associated with endoplasmic reticulum stress, intracellular trafficking, and immune function.

• 出版日期2011-7
• 单位吉林大学; 吉林医药学院