Age-Based Comparison of Human Dendritic Spine Structure Using Complete Three-Dimensional Reconstructions

作者：Benavides Piccione Ruth^*; Fernaud Espinosa Isabel; Robles Victor; Yuste Rafael; DeFelipe Javier

来源：Cerebral Cortex, 2013, 23(8): 1798-1810.

DOI：10.1093/cercor/bhs154

• cerebral cortex
• confocal
• intracellular
• Lucifer Yellow
• 3D reconstructions

Dendritic spines of pyramidal neurons are targets of most excitatory synapses in the cerebral cortex. Recent evidence suggests that the morphology of the dendritic spine could determine its synaptic strength and learning rules. However, unfortunately, there are scant data available regarding the detailed morphology of these structures for the human cerebral cortex. In the present study, we analyzed over 8900 individual dendritic spines that were completely 3D reconstructed along the length of apical and basal dendrites of layer III pyramidal neurons in the cingulate cortex of 2 male humans (aged 40 and 85 years old), using intracellular injections of Lucifer Yellow in fixed tissue. We assembled a large, quantitative database, which revealed a major reduction in spine densities in the aged case. Specifically, small and short spines of basal dendrites and long spines of apical dendrites were lost, regardless of the distance from the soma. Given the age difference between the cases, our results suggest selective alterations in spines with aging in humans and indicate that the spine volume and length are regulated by different biological mechanisms.

• 出版日期2013-8