A novel role of microtubular cytoskeleton in the dynamics of caspase-dependent Fas/CD95 death receptor complexes during apoptosis

作者：Doma Eszter; Chakrabandhu Krittalak; Hueber Anne Odile^*

来源：FEBS Letters, 2010, 584(5): 1033-1040.

DOI：10.1016/j.febslet.2010.01.059

• Apoptosis
• Microtubule
• Fas/CD95
• Lymphocyte

The activation of cysteine-aspartic proteases or caspases and the dynamic arrangement of cytoskeletal components are crucial during apoptosis. Here we describe the fate of Fas downstream of the FasL-induced internalization step, including formation of caspase-dependent SDS-stable Fas complexes, which is mediated by cytoskeleton integrity. We show, in particular, that following FasL treatment, the Fas lower aggregate complex can be co-immunoprecipitated with tubulin and an active form of caspase-8 and that this interaction contributes to the propagation of FasL-induced cell death. The importance of cytoskeletal components during FasL-induced apoptosis is highlighted by our detection of a pool of microtubule-associated Fas complexes.

• 出版日期2010-3-5