Development of Anonymous Nuclear Loci for Pterospora andromedea (Monotropoideae) Using Illumina and Ion Torrent Sequencing Data

作者：Dowie N J^*; Grubisha L C; Burton B A; Klooster M R; Miller S L

来源：Conservation Genetics Resources, 2017, 9(3): 371-373.

DOI：10.1007/s12686-017-0686-4

• Pterospora andromedea
• Rhizopogon
• Amylopogon
• Anonymous Nuclear Loci
• ANL
• Conservation
• Monotropoideae
• Ericaceae

The mycoheterotrophic plant Pterospora andromedea is an important species in understanding the evolution and ecology of highly specialized plant-fungal symbioses. Lineages within P. andromedea have been shown to engage in highly specific interactions with obligate fungal associates found in Rhizopogon subgenus Amylopogon. Unfortunately, evidence of highly specific interactions resulted from single locus analyses and this result requires further examination. If highly specific host-symbiont selectivity is present, then the lack of compatible partners may best explain the conservation status of P. andromedea. To better evaluate and support conservation efforts for P. andromedea, more molecular loci are necessary. 115 candidate loci were screened from Illumnia MiSeq and Ion Torrent next-generation sequencing and resulted in the development of four polymorphic anonymous nuclear loci. These loci will provide the means to critically examine host-symbiont specificity for P. andromedea lineages across the endemic range and will assist conservation initiatives.

• 出版日期2017-9