An ultrasensitive electrochemiluminescent immunosensor based on graphene oxide coupled graphite-like carbon nitride and multiwalled carbon nanotubes-gold for the detection of diclofenac

作者：Hu, Liuyi; Zheng, Jing; Zhao, Kang; Deng, Anping^*; Li, Jianguo^*

来源：Biosensors and Bioelectronics, 2018, 101: 260-267.

DOI：10.1016/j.bios.2017.10.043

• Electrochemiluminescent immunosensor
• Competitive inununoassay
• MWCNTs-AuNPs
• GO-g-C3N4
• Diclofenac
• Environmental analysis

In this study, a novel competition-type electrochemiluminescent (ECL) immunosensor for detecting diclofenac (DCF) was fabricated with graphene oxide coupled graphite-like carbon nitride (GO-g-C3N4) as signal probe for the first time. The ECL intensity of carboxylated g-C3N4 was significantly enhanced after being combined with graphene oxide (GO) which exhibited excellent charge-transport property. The sensing platform was constructed by multiwalled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs), which not only provided an effective matrix for immobilizing a large amount of coating antigen but also facilitated the electronic transmission rate to enhance the ECL intensity. Based on the synergistic effect of GO-g-C3N4 and MWCNTs-AuNPs composite, the proposed sensor showed high sensitivity, good stability, and wide linearity for the detection of DCF in the range of 0.005-1000 ng mL(-1) with a detection limit of 1.7 pg mL(-1). Furthermore, the developed immunoassay has been applied to real samples with satisfactory results. Therefore, this work provided a promising method for the detection of DCF and other small molecular compounds in the future.

• 出版日期2018-3-15
• 单位苏州大学