科研之友
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摘要
Introduction: The mdx(4cv) mouse is a common model to study Duchenne muscular dystrophy. The most used methodology to identify the genotype of these mice is Sanger DNA sequencing. Methods: Here, we provide a simple, costeffective alternative approach to identify the wild-type, heterozygous, or homozygous/hemizygous genotypes of these mice, using commonly available laboratory equipment and reagents. Results: Our technique exploits a restriction fragment length polymorphism that is generated by the point mutation found in exon 53 of mdx(4cv) mice. Conclusions: This technique can benefit laboratories that require complex breeding strategies involving mdx(4cv) mice.
- 出版日期2017-9
