Deep sequencing of transcriptome profiling of GSTM2 knock-down in swine testis cells

作者：Lv, Yuqi; Jin, Yi; Zhou, Yongqiang; Jin, Jianjun; Ma, Zhenfa; Ren, Zhuqing^*

来源：Scientific Reports, 2016, 6(1): 38254.

DOI：10.1038/srep38254

• CONCEPTUS INTERFERONS
• VASCULAR-PERMEABILITY
• EXPRESSION
• OSTEOPONTIN
• IMPLANTATION
• ENDOMETRIUM
• BOVINE
• IDENTIFICATION
• COACTIVATOR
• BLASTOCYSTS

Glutathione-S-transferases mu 2 (GSTM2), a kind of important Phase II antioxidant enzyme of eukaryotes, is degraded by nonsense mediated mRNA decay due to a C27T substitution in the fifth exon of pigs. As a reproductive performance-related gene, GSTM2 is involved in embryo implantation, whereas, functional deficiency of GSTM2 induces pre-or post-natal death in piglets potentially. To have some insight into the role of GSTM2 in embryo development, high throughput RNA sequencing is performed using the swine testis cells (ST) with the deletion of GSTM2. Some embryo development-related genes are observed from a total of 242 differentially expressed genes, including STAT1, SRC, IL-8, DUSP family, CCL family and integrin family. GSTM2 affects expression of SRC, OPN, and SLCs. GSTM2 suppresses phosphorylation of STAT1 by binding to STAT1. In addition, as an important transcription factor, STAT1 regulates expression of uterus receptive-related genes including CCLs, IRF9, IFITs, MXs, and OAS. The present study provides evidence to molecular mechanism of GSTM2 modulating embryo development.

• 出版日期2016-12-1
• 单位华中农业大学