Analysis of tomato gene promoters activated in syncytia induced in tomato and potato hairy roots by Globodera rostochiensis

作者：Wisniewska A^*; Dabrowska Bronk J; Szafranski K; Fudali S; Swiecicka M; Czarny M; Wilkowska A; Morgiewicz K; Matusiak J; Sobczak M; Filipecki M

来源：Transgenic Research, 2013, 22(3): 557-569.

DOI：10.1007/s11248-012-9665-4

• Gene promoter
• Globodera rostochiensis
• Hairy roots
• Nematode
• Tomato
• Syncytium

The potato cyst nematode (Globodera rostochiensis) induces feeding sites (syncytia) in tomato and potato roots. In a previous study, 135 tomato genes up-regulated during G. rostochiensis migration and syncytium development were identified. Five genes (CYP97A29, DFR, FLS, NIK and PMEI) were chosen for further study to examine their roles in plant-nematode interactions. The promoters of these genes were isolated and potential cis regulatory elements in their sequences were characterized using bioinformatics tools. Promoter fusions with the beta-glucuronidase gene were constructed and introduced into tomato and potato genomes via transformation with Agrobacterium rhizogenes to produce hairy roots. The analysed promoters displayed different activity patterns in nematode-infected and uninfected transgenic hairy roots.

• 出版日期2013-6