Simultaneous Determination of Methylxanthines and Cotinine in Human Plasma by Solid-Phase Extraction Followed by LC-MS-MS

作者：Bossi Rossana^*; Bech Bodil Hammer

来源：Spectroscopy, 2015, 31-34.

A multiresidue method has been developed and validated for the analysis of methylxanthines (caffeine and its metabolites) and cotinine in human plasma. The method involves off-line solid-phase extraction (SPE) and analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS-MS) with electrospray ionization (ESI) in positive mode. The developed and validated method was fast, selective, and convenient for the simultaneous determination of cotinine, caffeine, and its metabolites in human plasma. The method has been successfully applied to the analysis of 500 samples from pregnant women in a clinical study.

• 出版日期2015-2